Biochem/physiol Actions

ED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.

General description

Recombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ.50, 229.Ishii, Y., et al. 2008. J. Invest. Dermatol.128, 867.Kim, K.A., et al. 2008. Mol. Biol. Cell19, 2588.Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA104 14700.Nam, J.S., et al. 2007. Gene Expr. Patterns7, 306.Pemberton, T.J., et al. 2007. Dev. Dyn.236, 2245.Bergmann, C., et al. 2006. Am. J. Hum. Genet.79, 1105.Blaydon, D.C., et al. 2006. Nat. Genet.38, 1245.Nam, J.S., et al. 2006. J. Biol. Chem.281, 13247.

Packaging

25 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.

Reconstitution

Reconstitute to 200 µg/ml in PBS.

Warning

Toxicity: Standard Handling (A)